How HIV smuggles its genetic material into the cell nucleus

How HIV smuggles its genetic material into the cell nucleus

How HIV smuggles its genetic material into the cell nucleus
Artist’s impression shows how the HIV capsid penetrates the jelly-like permeability barrier of a nuclear pore. To smuggle its genome through this defense line into the cell nucleus, it has evolved into a molecular transporter. Credit: Johannes Pauly, Max Planck Institute for Multidisciplinary Sciences

Each year, about 1 million individuals worldwide become infected with HIV, the virus that causes AIDS. To replicate and spread the infection, the virus must smuggle its genetic material into the cell nucleus and integrate it into a chromosome.

Research teams led by Dirk Görlich at the Max Planck Institute for Multidisciplinary Science and Thomas Schwartz at the Massachusetts Institute of Technology (MIT) have now discovered that its capsid has evolved into a molecular transporter. As such, it can directly breach a crucial barrier, which normally protects the cell nucleus against viral invaders. This way of smuggling keeps the viral genome invisible to anti-viral sensors in the cytoplasm. Their study is published in Nature.

Forty years after the human immunodeficiency virus (HIV) was discovered as the cause of AIDS, we have therapies that effectively keep the pathogen under control, but there is still no cure. The virus infects certain immune cells and hijacks their genetic program in order to multiply and replicate its own genetic material. The infected cells then produce the next generation of viruses until they are finally destroyed. The immunodeficiency symptoms of AIDS result from the massive loss of immune cells that normally fight viruses and other pathogens.

To use the host cell’s resources, HIV must smuggle its genetic material through cellular defense lines into the cell nucleus. The nucleus, however, is closely guarded. Its nuclear envelope prevents unwanted proteins or harmful viruses from entering the nucleus and macromolecules from an uncontrolled escape. Yet, selected proteins can pass because the barrier is not hermetically sealed.

Thousands of tiny nuclear pores in the nuclear envelope provide a passageway. They control these transport processes with the help of importins and exportins—molecular transporters that capture cargoes with valid molecular “passcodes” and deliver them through the nuclear pore channel. A “smart” material turns these pores into one of nature’s most efficient sorting and transport machines.

‘Smart’ sorting in the nuclear pore

This “smart” material, called FG phase, is jelly-like and impenetrable for most macromolecules. It fills and blocks the nuclear pore channel. Importins and exportins, however, can pass through because their surfaces are optimized for sliding through an FG phase.

The cell’s border control in the FG phase happens extremely fast—within milliseconds. Likewise, its transport capacity is enormous: A single nuclear pore can transfer up to 1,000 transporters per second through its channel. Even with such a high traffic density, the barrier of nuclear pores remains intact and keeps suppressing unwanted border crossings. HIV, however, subverts this control.

Smuggled genetic material

“HIV packages its genome into a capsid. Recent evidence suggests that the genome stays inside the capsid until it reaches the nucleus, and thus also when passing the nuclear pore. But there is a size problem,” Thomas Schwartz of MIT explains. The central pore channel is 40 to 60 nanometers wide. The capsid has a width of about 60 nanometers and could just squeeze through the pore.

However, a normal cellular cargo would still be covered by a transporter layer that adds at least another ten nanometers. The HIV capsid would then be 70 nanometers wide—too big for a nuclear pore. “Nevertheless, cryo-electron tomography has shown that the HIV capsid gets into the nuclear pore. But how this happens has been so far a mystery in HIV infection,” says Max Planck Director Görlich.

More information:
Liran Fu et al, HIV-1 capsids enter the FG phase of nuclear pores like a transport receptor, Nature (2024). DOI: 10.1038/s41586-023-06966-w

Provided by
Max Planck Society


Citation:
How HIV smuggles its genetic material into the cell nucleus (2024, January 25)
retrieved 25 January 2024
from https://phys.org/news/2024-01-hiv-smuggles-genetic-material-cell.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.

Source link

Leave a Reply

Your email address will not be published. Required fields are marked *