Illuminating tiny proteins in living cells using single-residue labeling tags

IMAGE: Universal single-residue terminal labels for fluorescent live cell Imaging of microproteins view more  Credit: Simon Elsasser Thirty years ago, the cloning of the green fluorescent protein GFP, together with genetic engineering tools, revolutionized biology by enabling researchers to fuse a fluorescent ‘beacon’ to any protein of interest so that it can be directly observed in […]

Illuminating tiny proteins in living cells using single-residue labeling tags

Universal single-residue terminal labels for fluorescent live cell Imaging of microproteins. Credit: Simon Elsasser SciLifeLab Fellow Simon Elsässer laboratory at Karolinska Institutet reports a method, which allows fluorescent tagging of proteins with the small perturbation—a single amino acid—added genetically on either end of a (micro)protein of interest. The method is termed Single-residue Terminal Labeling (STELLA). […]